Sources and Remedies of Phototoxicity in Live Cell Microscopy
نویسندگان
چکیده
منابع مشابه
Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range
Light-induced toxicity is a fundamental bottleneck in microscopic imaging of live embryos. In this article, after a review of photodamage mechanisms in cells and tissues, we assess photo-perturbation under illumination conditions relevant for point-scanning multiphoton imaging of live Drosophila embryos. We use third-harmonic generation (THG) imaging of developmental processes in embryos excite...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 2000
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500063434